Specific Identification of Swine Nucleic Acid Sequence Using Polymerase Chain Reaction (Pcr)

Pork consumption is prohibited in some religions. Therefore, religious people are adamant about importing processed food, which may contain or has been contaminated with pork or swine-derived products. In Sudan, no reliable assays exist for detecting the presence of pork in processed food. Currently, regulatory officials rely on a paper trail for this verification. To address the void in...

Development and Evaluation of Pcr Assays for Detection of Animal-Derived Products in Processed Food and Animal Feed Concentrates

Nested (nPCR) and semi-nested polymerase chain reaction assays for specific identification of animal-derived products in processed food and in animal feed concentrates were developed and evaluated. The mitochondrial cytochrome-b (mtcyt-b) gene was used as a target DNA for PCR amplification. Pairs of primers derived from highly conserved regions of mtcyt-b gene were used for the PCR assays in...